N content-related indicators in shoots and roots of STWs, their diploid parents and NTW under either NN or LN conditions for 10 and 30 days

The indicators include total N content, total N transfer rate, nitrate (NO₃⁻) content, and ammonium (NH₄⁺) content, as well as the relative changes (RC) of all N content indicators across treatments and time points.

Statistical significance of differences was evaluated using two methods based on indicator types: For total N transfer rate, NO₃⁻ content (main panel) and NH₄⁺ content: Statistical significance between groups was determined by Student’s t-test, with significance levels marked as *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 in the associated visualization files. For total N content, NO₃⁻ content under LN-30d (enlarged inset view) and RC of N content indicators: Significant differences across genotypes were tested via one-way analysis of variance (ANOVA) followed by Tukey HSD tests.