Data · dataset · 2026
<p>The <i>nhre3</i> gene responds to and promotes GCRV infection.</p>
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<p>(A) qPCR analysis of <i>nhre3</i> gene expression in the gill, hepatopancreas, kidney, and intestine of rare minnow on days 0, 3, and 7 post-GCRV infection.
Description
(B) qPCR analysis of <i>nhre3</i> gene expression in the gill, hepatopancreas, kidney, and intestine of grass carp on days 0, 3, and 7 post-GCRV infection. (C) qPCR analysis of <i>nhre3</i> gene expression in CIK cells at 0, 6, 12, 24, and 36 h post-GCRV infection.
(D) Western blot analysis of NHRE3 protein and GCRV VP5 protein levels in CIK cells at 0, 6, 12, 24, and 36 h post-GCRV infection. (E) qPCR analysis of <i>ifn1</i>, <i>isg15</i>, and <i>nhre3</i> gene expression levels in CIK cells treated with the activator Vadimezan (100μg/ml) after 24h, DMSO treatment as the control group. (F) CIK cells overexpressing <i>nhre3</i> for 24 h were subsequently challenged with GCRV.
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The expression level of the GCRV S10 segment was measured using qPCR at 8, 16, and 24 h post-challenge. Cells transfected with an empty vector served as the control group. (G) CIK cells overexpressing <i>nhre3</i> for 24 h were subsequently challenged with GCRV.
The expression level of the GCRV NS1 segment was measured using qPCR at 8, 16, and 24 h post-challenge. Cells transfected with an empty vector served as the control group. (H) CIK cells were transfected with 0, 1, or 2 μg of <i>nhre3</i> overexpression plasmid for 24 h and subsequently challenged with GCRV.
The expression levels of GCRV VP5 and NHRE3 proteins were measured via western blot at 24 h post-challenge. (I) CIK cells overexpressing <i>nhre3</i> for 24 h were subsequently challenged with GCRV. Cytopathic effects (CPE) were assessed using crystal violet staining at 24 h post-challenge.
All experiments were repeated for at least three times with similar results.</p>
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Where it is published
- DOI doi.org/10.1371/journal.ppat.1014544.g003 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
Provenance · 1 source records, 17 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34026292 | 10 d ago | JSON v1 |
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| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
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| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |