Table · dataset · 2026
Dataset related to "Split inteins for generating combinatorial non-ribosomal peptide libraries"
Listed in Edmond
HR-LC–MS/MS data for 324 combinatorial split-intein NRPS variants.
Description
This dataset contains raw high-resolution liquid chromatography–tandem mass spectrometry (HR-LC–MS/MS) measurements for 324 Escherichia coli DH10B::mtaA strains expressing combinatorially assembled non-ribosomal peptide synthetases (NRPSs). The library comprises all combinations of six initiation units, nine elongation units and six termination units generated using an orthogonal split-intein platform.
The accompanying table links the raw measurement filenames to the corresponding NRPS constructs and detected peptide products. It includes construct identifiers, peptide sequences, molecular formulas, SMILES representations, exact masses, expected and measured [M+H]+ values, and mass deviations in ppm. Of the 324 NRPS variants, 173 produced at least one expected peptide, with 258 linear or cyclic peptide products identified in total.
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For detailed information on library construction, cultivation, peptide extraction, HR-LC–MS/MS analysis, please the related publication “Split inteins for generating combinatorial non-ribosomal peptide libraries”.
Links
Where it is published
- Dataverse dataset page edmond.mpg.de/dataset.xhtml?persistentId=doi%3A10.17617%2F3.IBKIKF ↗
landing page · from edmond mpdl mpg de
- DOI doi.org/10.17617/3.ibkikf ↗
DOI / persistent id · from edmond mpdl mpg de
Catalogue records · 1
- Dataverse API edmond.mpg.de/api/datasets/:persistentId/?persistentId=doi%3A10.17617%2F3.IB… ↗
metadata API · from edmond mpdl mpg de
Topics
- From keywords
- Astronomy & Astrophysics · Chemistry · Chemistry · Computer Science & AI · Earth & Environmental Science · Economics & Finance · Engineering · Humanities · Life Sciences · Life Sciences · Materials Science · Medicine & Health · Ocean & Atmospheric Science · Physics · Social Science · Synthetic biology · Synthetic biology
- Inferred from text
- Mass spectrometry 75% · Tabular 65%
Provenance · 2 source records, 29 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Edmond | doi:10.17617/3.IBKIKF | 9 d ago | JSON v1 |
| Edmond | doi:10.17617/3.IBKIKF | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:field:310113 | mapping · edmond mpg de | vocabulary-mapper@1.0.0 | keywords['synthetic biology'] |
| concepts[field].anzsrc:field:310113 | mapping · edmond mpdl mpg de | vocabulary-mapper@1.0.0 | keywords['synthetic biology'] |
| concepts[field].dataverse_subject:biology | source · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].dataverse_subject:biology | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | /subjects |
| concepts[field].dataverse_subject:chemistry | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | /subjects |
| concepts[field].dataverse_subject:chemistry | source · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:astronomy | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:chemistry | mapping · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:chemistry | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:computer-science-ai | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:earth-environmental | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:economics-finance | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:engineering | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:humanities | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:materials-science | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:ocean-atmospheric | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:physics | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[field].local:field:social-science | mapping · edmond mpg de | connector:edmond_mpg_de@1.0.0 | /subjects |
| concepts[modality].local:modality:mass-spectrometry | enrichment · edmond mpdl mpg de | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:tabular | enrichment · edmond mpdl mpg de | keyword-concept-rules@1.0.0 | title+description (65%) |
| created_date | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | |
| description | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | /description |
| publication_date | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | |
| title | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | /name |
| updated_date | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 | |
| version_label | source · edmond mpdl mpg de | connector:edmond_mpdl_mpg_de@1.0.0 |