Structure · dataset · 2026
Mapping ToxCast/Tox21 Assay Endpoints to Key Characteristics of Carcinogens to Support Cancer Hazard Evaluation
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.1021/ehp.6c00478.s002
The Environmental Protection Agency’s Toxicity Forecaster (ToxCast) and the Toxicity Testing in the 21st Century federal consortium (Tox21) programs provide vast high-throughput screening data on thousands of chemicals, capturing a range of molecular and cellular responses relevant to toxicity.
Description
However, their application to cancer hazard identification requires a structured framework. The ten key characteristics of carcinogens (KCCs) offer a systematic approach for organizing this mechanistic evidence.
We present an updated, comprehensive mapping of 1,570 ToxCast/Tox21 assay endpoints to the KCCs, developed through an expert-driven consensus process. A novel tiering approach was introduced to characterize each assay’s level of mechanistic relevance: Tier A indicates a direct effect linked to a KCC, while Tier B signifies an indirect or downstream effect. Additionally, we incorporated response directionality to correctly identify events contributing to, rather than merely associated with, a KCC.
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Of the 1,570 assay endpoints available in ToxCast/Tox21, 838 assay endpoints (53%) were mapped to the KCCs with some assay endpoints mapping to multiple KCCs. The majority of mapped assay endpoints were associated with KCC8 (modulates receptor-mediated effects) and KCC10 (alters cell proliferation, cell death, or nutrient supply). Some assay endpoints were mapped on different KCCs depending on the directionality of the response.
No assay endpoints were mapped to KCC1 (is electrophilic or can be metabolically activated to electrophiles) or KCC9 (causes immortalization). This publicly available tiered mapping provides a crucial resource for interpreting high-throughput screening data in the context of carcinogenesis. It enables researchers and risk assessors to more systematically utilize such data for cancer hazard evaluation and chemical prioritization.
Links
Where it is published
- DOI doi.org/10.1021/ehp.6c00478.s002 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Cancer · Cancer · Cancer · Chemistry · Chemistry · Chemistry · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Genetics · Inorganic chemistry · Inorganic chemistry · Inorganic chemistry · Life Sciences · Life Sciences · Life Sciences · Medicine & Health · Medicine & Health · Medicine & Health
Provenance · 3 source records, 26 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34033335 | 11 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34033335 | 11 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34033335 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3402 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Inorganic Chemistry'] |
| concepts[field].anzsrc:group:3402 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Inorganic Chemistry'] |
| concepts[field].anzsrc:group:3402 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Inorganic Chemistry'] |
| concepts[field].local:field:chemistry | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |