Spatiotemporal metabolic networks in a preclinical model of pancreatic cancer and associated cachexia

Our scripts were made on MATLAB for macOS. Some modifications for Windows may need to be made. INSTALLATION GUIDE: MATLAB can be downloaded here: mathworks.com/help/install/ug/install-products-with-internet-connection.html -Follow standard instructions by MathWorks.

Time to install: about 10 minutes INSTRUCTIONS FOR USE: Codes are available for 3D scatterplots, Dandelion Plots, Umbrella Plots, and Prediction Models. Codes in Text files can be downloaded for viewing in Text editor apps, but must be copied into MATLAB in order to run. To run each MATLAB script: Download data files from same folder.

Copy directory of the data files to the top of the script as prompted. Run script. Graphs/figures from the manuscript will be generated.

Expected Run time: <10 minutes for most scripts, up to an hour for prediction models. Note: models that include multiple iterations/random selection of subsampling will yield slightly different results each time it is run. A representative example was used in the manuscript. "Interactive" Plots are in sub-folders in 3DScatterplots, CorrelationPlots, DandelionPlots, and UmbrellaPlots.

All interactive plots have a ".fig" extension which also requires MATLAB to open and interact. 3D scatterplots can be rotated, and data tips show each metabolite name and coordinates. Correlations plots can be zoomed in/out and panned. Additionally, Heatmaps are available as .eps files, and can be downloaded and viewed.

Source Files include: -Metabolomics for each method per tissue (not normalized or processed) -Mouse model characterization data -Tracing source data -Fold-change and p-values for all volcano plots