In vivo online monitoring of intracellular lipid accumulation in Ustilago maydis

Accordingly, we aimed at developing an intrinsic reporter that is suitable to track oil formation even in larger cultures. From three tested candidates, the potential delta(24)-sterol C-methyltransferase Erg6 turned out as the best reporter. Fluorescence microscopy confirmed its localization at the LD membrane.

After optimization, Erg6 fused to mKate2 expressed from a promoter derived from glycolipid biosynthesis showed a good correlation of fluorescence with oil accumulation. Time course experiments in micro-cultivators demonstrated that the fluorescence read-out can be used to track oil formation starting at the onset of nitrogen limitation to approximate the LD amount. In essence, our study introduces a biosensor for oil monitoring, that can easily be transferred to other oleaginous yeasts. hosted on: git.nfdi4plants.org/projects/3204