Transcriptome profiling of Arabidopsis thaliana PsbS and β-carbonic anhydrase overexpression lines in response to bicarbonate fertilization

The photosystem II subunit PsbS plays a central role in non-photochemical quenching, while β-carbonic anhydrases contribute to CO₂ conversion and pH homeostasis in chloroplasts. To investigate the potential crosstalk between these components, we performed RNA-seq analysis of Arabidopsis thaliana lines overexpressing PsbS alone or in combination with β-carbonic anhydrase genes (βCA1 and βCA2).

Wild-type (Col-0), the npq4 mutant, and transgenic overexpression lines were grown under controlled conditions and treated either with water or bicarbonate fertilization. Transcriptome profiling was performed using Illumina NovaSeq sequencing of poly(A)+ RNA libraries prepared from total RNA. The dataset contains three biological replicates for each genotype and treatment combination.