Data · dataset · 2025
Alteration of Proteomes in First-generation Cultures of Bacillus pumilus Spores Exposed to Outer Space
Listed in NASA Data Portal
Bacillus pumilus SAFR-032 was originally isolated from the Jet Propulsion Lab Spacecraft Assembly Facility and thoroughly characterized for its enhanced resistance to UV irradiation and oxidative stress.
Description
This unusual resistance of SAFR-032 is of particular concern in the context of planetary protection and calls for development of novel disinfection techniques to prevent extraterrestrial contamination. Previously spores of SAFR-032 were exposed for 18 xe2 x80 x89months to a variety of space conditions on board the International Space Station to investigate their resistance to Mars-like conditions and space travel.
Here proteomic characterization of vegetative SAFR-032 cells from space-surviving spores is presented in comparison to a ground control. Vegetative cells of the first passage were processed and subjected to quantitative proteomics using tandem mass tags. Approximately 60% of all proteins encoded by SAFR-032 were identified and 301 proteins were differentially expressed among the strains.
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We found that proteins predicted to be involved in carbohydrate transport/metabolism and energy production/conversion had lower abundance than those of the ground control. For three proteins we showed that the expected metabolic activities were decreased as expected with direct enzymatic assays. This was consistent with a decrease of ATP production in the space-surviving strains.
The same space-surviving strains showed increased abundance of proteins related to survival growth advantage and stress response. Such alterations in the proteomes provide insights into possible molecular mechanisms of B. pumilus SAFR-032 to adapt to and resist extreme extraterrestrial environments.
Links
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- genelab-data.ndc.nasa.gov /genelab/accession/GLDS-259 ↗
Alteration of Proteomes in First-generation Cultures of Bacillus pumilus Spores Exposed to Outer Space
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Where it is published
- NASA Data Portal dataset page data.nasa.gov/dataset/alteration-of-proteomes-in-first-generation-cultures-o… ↗
landing page · from data nasa gov
Catalogue records · 1
- CKAN API data.nasa.gov/api/3/action/package_show?id=7768676b-1c6e-4690-a4fb-1a7a401b9… ↗
metadata API · from data nasa gov
Topics
- From keywords
- Life Sciences · Mass spectrometry
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Data Portal | 7768676b-1c6e-4690-a4fb-1a7a401b96ea | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:life-sciences | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | mapping · data nasa gov | vocabulary-mapper@1.0.0 | keywords['mass-spectrometry'] |
| created_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| description | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /notes |
| license_text | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| publication_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| title | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /title |
| updated_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 |