C. elegans excretory/secretory proteome

This data was generated at Génome Québec (QC, Canada) in 2011. Worms were synchronized on agar plates, washed extensively, and incubated in M9 buffer for 4 h.

The collected excretory/secretory proteins were separated by SDS-PAGE on a 7–15% gradient acrylamide gel, and stained with Coomassie Brilliant Blue G. The entire lane was subjected to automated band excision to generate 13 contiguous bands which were subjected to reduction, cysteine-alkylation, and in-gel tryptic digestion. MS/MS raw data (Q-TOF micro (Waters Micromass, Manchester, UK)) were transferred from the Q-TOF Microcomputer to a server and automatically manipulated for generation of peaklists (MGF files) by employing Distiller version 2.3.2.0 (matrixscience.com/distiller.html) with peak picking parameters set at 5 for Signal Noise Ratio (SNR) and at 0.4 for Correlation Threshold (CT).