Data · dataset · 2014
Kinetoplast and nuclear DNA staining in Leishmania mexicana
Listed in DataCite
Kinetoplast and nuclear DNA in kinetoplastids bind different DNA stains with different affinities.
Description
Staining of Leishmania mexicana promastigotes was optimised for five DNA stains (DAPI, propidium iodide, SYTO16, DRAQ5 and hoechst 33342). Maximum brightness (maximum pixel intensity) and total signal (sum pixel intensity) of the kinetoplast and nucleus were quantified from widefield epifluorescence micrographs for each stain.
Two stains were analysed from methanol fixed cells mounted in glycerol DABCO: DAPI and propidium iodide. The others were analysed from live cells in PBS. Propidium iodide staining included an RNase A digestion.
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This resource lets the relative contribution of kinetoplast and nuclear DNA be determined from signal intensity data from fluorescence micrographs and flow cytometry. This is a useful resource if working with the methods in one of my previous papers; see links.
Links
Where it is published
- Repository landing page figshare.com/articles/dataset/Kinetoplast_and_nuclear_DNA_staining_in_Leish… ↗
landing page · from DataCite
- Repository landing page figshare.com/articles/dataset/Kinetoplast_and_nuclear_DNA_staining_in_Leish… ↗
landing page · from DataCite
- DOI doi.org/10.6084/m9.figshare.1211896 ↗
DOI / persistent id · from DataCite
- DOI doi.org/10.6084/m9.figshare.1211896.v1 ↗
DOI / persistent id · from DataCite
Documentation and papers
Catalogue records · 4
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.1211896.v1 ↗
metadata API · from DataCite
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.1211896 ↗
metadata API · from DataCite
- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.1211896.v1 ↗
catalogue entry · from DataCite
- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.1211896 ↗
catalogue entry · from DataCite
Topics
- Stated by source
- Health sciences · Health sciences
Provenance · 2 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.1211896 | 14 d ago | JSON v1 |
| DataCite | 10.6084/m9.figshare.1211896.v1 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| byte_size | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:health-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:health-sciences | source · DataCite | connector:datacite@1.0.0 | |
| created_date | source · DataCite | connector:datacite@1.0.0 | |
| description | source · DataCite | connector:datacite@1.0.0 | /data/attributes/descriptions |
| license | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| publication_date | source · DataCite | connector:datacite@1.0.0 | /data/attributes/dates |
| title | source · DataCite | connector:datacite@1.0.0 | /data/attributes/titles/0/title |
| updated_date | source · DataCite | connector:datacite@1.0.0 |