Omics · study · 2026
Derivation of elephant induced pluripotent stem cells [short-read RNA-seq]
Listed in NCBI GEO
The crisis of biodiversity loss in the present era requires new tools for studying non-model organisms.
Description
Elephants are both an endangered species and excellent models for studying complex phenotypes including body size, social behavior, and longevity. Here we report here the first derivation of (Elephas maximus) induced pluripotent stem cells (emiPSCs).
We achieved emiPSCs using two approaches: (1) a two-step process of chemical-media induction and colony selection followed by overexpression of elephant transcription factors; and (2) a one-step process with the same transgenes but without the chemical induction step and including an additional HRAS mutant transgene, HRASG12V. For both protocols, we inhibited TP53 expansion genes, which are hypothesized to confer unique cancer resistance in elephants.
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To confirm their reprogrammed state, we generated a functional genomics catalog of the modified cells with RNA-seq, ATAC-seq, and single-molecule direct RNA-sequencing. While these emiPSCs are transgene-dependent, we inactivated them to differentiate emiPSCs into all three germ layers via tri-lineage differentiation, embryoid body generation, and direct differentiation into putative cell types from all three layers. These methods will open new frontiers for cellular models of non-model organisms, including for genetic rescue and conservation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300277 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300277 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1280018 ncbi.nlm.nih.gov/bioproject/PRJNA1280018 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE300277 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |