Supplementary file 1_Polyketide synthase gene clusters in the ericoid mycorrhizal fungus Oidiodendron maius: genome-wide prediction, expression profiling, and functional characterization of knockout mutants.zip

However, their roles in mycorrhizal fungi remain poorly understood. In this study, we investigated the repertoire and potential functions of polyketide synthases (PKSs) in the ericoid mycorrhizal fungus Oidiodendron maius.</p>Methods and Results<p>Genome-wide analyses identified 45 PKS-coding genes and 59 predicted biosynthetic gene clusters associated with secondary metabolite production.

Transcriptomic analyses revealed that several PKS genes were differentially regulated during symbiosis with Vaccinium myrtillus and under cadmium exposure. Among these, the gene OmPKS197601 was strongly upregulated under both conditions and was selected for functional characterization through Agrobacterium tumefaciens-mediated gene disruption. Three independent OmPKS197601 knockout mutants were generated and evaluated for their ability to establish mycorrhizal symbiosis and tolerate cadmium and zinc stress.