Data · collection · 2016
RNA sequence data from krill CO2 exposure experiment
Listed in NASA Earthdata CMR
We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels.
Description
We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm).
These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages
Read the rest (1 more)
- 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).
Links
Get the data
- Request point for the data data.aad.gov.au/aadc/portal/download_file.cfm?file_id=4618 ↗
download · from NASA CMR
Where it is published
- DOI doi.org/10.4225/15/5775d60f1cb0d ↗
DOI / persistent id · from NASA CMR
Documentation and papers
- data.aad.gov.au /aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA ↗
Citation reference for this metadata record and dataset.
documentation · from NASA CMR
- Public information for AAS project AAS_4015 secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS… ↗
project · from NASA CMR
Catalogue records · 2
- CMR UMM-JSON cmr.earthdata.nasa.gov/search/concepts/C1292610828-AU_AADC.umm_json ↗
metadata API · from NASA CMR
- CMR record cmr.earthdata.nasa.gov/search/concepts/C1292610828-AU_AADC.html ↗
catalogue entry · from NASA CMR
Topics
- Stated by source
- Automated DNA Sequencer · Carbon Dioxide · Euphausiids Krill · LABORATORY
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Earthdata CMR | C1292610828-AU_AADC | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[instrument].gcmd_instrument:ads | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/biological-classification/animals-invertebrates/arthropods/crustaceans/euphausiids-krill | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/oceans/ocean-chemistry/carbon-dioxide | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · NASA CMR | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NASA CMR | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[platform].gcmd_platform:laboratory | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| description | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/Abstract |
| license_text | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| publication_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| spatial | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/SpatialExtent |
| temporal | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/TemporalExtents |
| title | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/EntryTitle |
| version_label | source · NASA CMR | connector:nasa_cmr@1.0.0 |