Data · dataset · 2021
Nature Communications Biology RADAR - Feb 2021
Listed in RADAR and RADAR4Memory — shown once because both records carry DOI 10.22000/400
Black pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine.
Description
Its pungent perception is due to the interaction of its major compound, 1-piperoyl-piperidine (piperine) with the human TRPV-1 or vanilloid receptor. We now identified the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper fruits.
This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits has a rather promiscuous substrate specificity combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase.
Read the rest (1 more)
Recombinant piperine and piperamide synthases are members of a small BAHD-gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic amides piperamides based on a wide array CoA-donors and amine-derived acceptors offering widespread applications.
Links
Where it is published
- DOI doi.org/10.22000/400 ↗
DOI / persistent id · from radar service eu de
Catalogue records · 1
- OAI-PMH record radar-service.eu/oai/OAIHandler?verb=GetRecord&metadataPrefix=oai_dc&identifier… ↗
metadata API · from radar service eu de
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- RNA sequencing 65%
Provenance · 2 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| RADAR | 10.22000/400 | 12 d ago | JSON v1 |
| RADAR4Memory | 10.22000/400 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · radar service eu de | connector:radar_service_eu_de@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · radar4memory radar service eu | connector:radar4memory_radar_service_eu@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · radar service eu de | connector:radar_service_eu_de@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · radar service eu de | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · radar service eu de | connector:radar_service_eu_de@1.0.0 | /metadata/dc/description |
| license | source · radar service eu de | connector:radar_service_eu_de@1.0.0 | /metadata/dc/rights |
| publication_date | source · radar service eu de | connector:radar_service_eu_de@1.0.0 | |
| title | source · radar service eu de | connector:radar_service_eu_de@1.0.0 | /metadata/dc/title |