Image repository from : Subcellular nanoparticle trafficking investigated with label-free, live cell imaging

Delivery kinetics can also be modified by altering the surface chemistry of the nanoparticle carrier. However, a gap remains in our understanding of the cellular mechanisms underlying nanoparticle uptake and subcellular trafficking kinetics.

A comprehensive understanding of nanoparticle-cell interactions considering both the nanocarrier and the drug cargo has been challenging, partly due to technological limitations. Here, we present a robust long-term imaging workflow as a novel tool to study live-cell dynamics and nanoparticle delivery. We show how the integration of holography, tomography, and fluorescence microscopy enhances the capacity to study living, adherent cells in vitro label-free with further applications in assessing nanoparticle uptake and delivery kinetics over time.