Pathological mechanisms of long non-coding RNA FOXD2-AS1 promoting osteosarcoma mediated by the COI-1/OCN/ Runx-2 signaling pathway

OS Osteosarcoma  Raw data: GSE14359, 2 normal samples, 10 osteosarcoma samples (excluding lung metastasis tissue samples)  GSE14359_series_matrix.txt.gz: Normalized matrix and clinical information  GPL96.annot.gz: Platform annotation file  Differential expression analysis results:  limma-Test.txt: Differential expression analysis results for all genes using the limma package  DEG.txt: Differentially expressed genes identified by limma package (|logFC| > 1 and adj.P.Val < 0.05)  DEG-volcano: Volcano plot of differentially expressed genes  Differentially expressed gene enrichment analysis:  DEG-GO.csv: GO enrichment results file  DEG-KEGG.csv: KEGG pathway enrichment results file  DEG-GO-5: Top 5 pathways in BP, CC, and MF (based on q-value)  DEG-GO-10: Top 10 pathways in BP, CC, and MF  DEG-KEGG-10: Top 10 KEGG pathways  Co-expression analysis:  cor_lnc-mRNA.txt: Co-expression analysis results between differentially expressed lncRNAs and mRNAs (pval < 0.05 and cor > 0)  ceRNA network construction:  Pairs of differentially expressed lncRNAs and mRNAs with pval < 0.01 and cor > 0.9 were selected to construct the ceRNA network (175 pairs, 6 lncRNAs, 169 mRNAs)  Prediction of miRNA–mRNA interaction pairs:  miRNA–mRNA interaction pairs validated by Luciferase reporter assays from the miRTarget database in the multiMiR package  Prediction of miRNA–lncRNA interaction pairs:  miRNA–lncRNA interaction pairs downloaded from ENCORI database based on hg38 genome (originally from StarBase database)  By integrating the filtered lncRNAs and mRNAs, interacting miRNAs were identified and intersected to generate the ceRNA network  ceRNA network files:  edges.txt: Interaction file  nodes.txt: Gene type file  ceRNA network visualization:edge.pdfedges.pdf