Sequencing of TP53 in cisplatin-treated and cisplatin-resistant cells

In addition, there were untreated A549 and A549/DDP cells.Each sample was 1×106 cells before DNA extraction to ensure enough tissue to be processed. The primer pairs were: TP53-1,5'-GTCCCTCTCTGATTGTCTTTTCC-3' and 5'-ACTGACAGGAAGCCAAAGGG-3'; TP53-2,5'-TGTTTGTTTCTTTGCTGCCG-3' and 5'-CAAATAAGCAGCAGGAGAAAGC-3'; TP53-3,5'-GAGACCATCCTGGCTAACGG-3' and 5'-ACACCATCGTAAGTCAAGTAGCATC-3'.

DNA was isolated by a Ezup Column Animal Genomic DNA Purification Kit. Samples were re-suspended in buffer CL, proteinase K, CW1 Solution, CW2 Solution, and CE Buffer for further purification and PCR. After DNA purity and concentration were determined, 1 µL template DNA, 2 µL primer sequences, and 2.5 µL of Taq Buffer (with MgCl2) (10×) were mixed together for the PCR cycle.