Raw data of DAP-Seq of GiMYB76

A total of 5 μg genomic DNA was sheared to ~200 bp fragments; the repaired ends were ligated Illumina-based sequencing adaptors to construct an adaptor-ligated DNA library.The ORF sequence of GiMYB76 was fused with the Halo affinity tag and expressed in vitro using TNT® SP6 Coupled Wheat Germ Extract System (Promega, Fitchburg, WI, USA).

The fusion protein was bound to magnetic Halo Tag ligand (chloroalkane) beads, isolated from the expression mixture, and verified by Western blotting with anti-Halo Tag antibody (Promega), with nonspecific proteins were washed away. HaloTag-GiMYB76 fusion proteins were incubated with 500 ng of adaptor-ligated genomic DNA library at 30 ℃ for 2 h. Unbound DNA fragments were removed by washing, and samples were heated at 98 ℃ for 10 min to release GiMYB76 bound DNA The recovered DNA was amplified by PCR using indexed TruSeq primers.