Exploring the therapeutic mechanism of Patrinia villosa on metabolic dysfunction-associated steatohepatitis in mice based on transcriptomics and molecular docking

A high-fat and high sugar diet combined with fructose water was used to establish a MASH mouse model. Forty C57BL/6J mice were randomly divided into a normal control group, a model group, a low-dose group of Scutellaria baicalensis (0.17 g/kg), a high-dose group of Scutellaria baicalensis (0.34 g/kg), and an obeticolic acid group, and treated continuously for 8 weeks.

Detect serum liver function indicators, blood lipid levels, oxidative stress indicators, and inflammatory factors; HE staining and Oil Red O staining were used to observe the pathological changes in the liver; Transcriptome sequencing analysis of differentially expressed genes; Western blot validation of core target protein expression; Molecular docking predicts the binding mode between active ingredients and target proteins.