Omics · dataset · 2025
Tiled-amplicon sequencing and variant analysis of p6.9 rBEV genomes
Listed in Borealis
Control and CRISPR-Cas9 targeted baculovirus (p6.9GFP rBEV) genomes, upon plasmid-based delivery of sgRNA into Sf9-Cas9 cells, were extracted and sequenced via tiled-amplicon sequencing.
Description
Variant analysis of the tiled-amplicon sequenced control and targeted p6.9GFP rBEV genomes was performed to confirm CRISPR mutations. Variant analysis of the shotgun sequenced p6.9GFP rBEV stock was also performed to investigate variant conservation upon viral propagation in insect cell culture.
Links
Where it is published
- Dataverse dataset page borealisdata.ca/dataset.xhtml?persistentId=doi%3A10.5683%2FSP3%2FYEECMU ↗
landing page · from borealisdata ca
- DOI doi.org/10.5683/sp3/yeecmu ↗
DOI / persistent id · from borealisdata ca
Catalogue records · 1
- Dataverse API borealisdata.ca/api/datasets/:persistentId/?persistentId=doi%3A10.5683%2FSP3%2… ↗
metadata API · from borealisdata ca
Topics
- Stated by source
- Engineering · Medicine, Health and Life Sciences
- From keywords
- Computer Science & AI · Engineering · Genome sequencing · Life Sciences · Medicine & Health · Sequencing
- Inferred from text
- Bioinformatics and computational biology 72%
Provenance · 1 source records, 15 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Borealis | doi:10.5683/SP3/YEECMU | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3102 | enrichment · borealisdata ca | taxonomy-embedding@1.1.0 | title+keywords+description (72%) |
| concepts[field].dataverse_subject:engineering | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:computer-science-ai | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:engineering | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[modality].local:modality:genome-sequencing | mapping · borealisdata ca | vocabulary-mapper@1.0.0 | keywords['Whole genome sequencing'] |
| concepts[modality].local:modality:sequencing | mapping · borealisdata ca | vocabulary-mapper@1.0.0 | keywords['Next-generation sequencing'] |
| created_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| description | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /description |
| publication_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| title | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /name |
| updated_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| version_label | source · borealisdata ca | connector:borealisdata_ca@1.0.0 |