Data · dataset · 2026
QUB-1383, a novel bioactive peptide from the defensive skin secretion of the edible frog (Pelophylax kl. esculentus)
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32862668.v1
In this study, a novel peptide named QUB-1383 was successfully isolated from the skin secretion of the edible frog (Pelophylax kl. esculentus) via “shotgun” cloning of the precursor-encoding cDNA.
Description
Afterwards, the predicted mature peptide was chemically synthesised by solid-phase peptide synthesis and its structure confirmed via MALDI-TOF mass spectrometry. Then the crude synthesised peptide was purified via RP-HPLC.
Several bioassays including antimicrobial assays, haemolytic assays and trypsin inhibitor assays were conducted to ascertain its bioactivities. The synthetic QUB-1383 not only possessed potent antimicrobial activity against C.albicans, S. aureus and E. coli with minimal inhibitory concentrations at 4 μM, 2 μM and 16 μM, respectively. However, it also displayed relatively strong haemolytic activity with EC50 value at 39.64 μM. QUB-1383 also exerted weak inhibitory effect to trypsin, with a Ki value of 20.74 ± 6.87 μM. <br>Due to the potent antimicrobial activity of QUB-1383, this peptide could be considered as a candidate of novel antibiotics after target-modification to minimise its haemolytic effect.
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Since the working concentration of QUB-1383 to inhibit trypsin was too high, the trypsin inhibitory activity could not be considered for further development.
Links
Where it is published
- DOI doi.org/10.17034/32862668.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Mass spectrometry 75%
Provenance · 3 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32862668 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32862668 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32862668 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |