Supplementary material from "Incubation under climate warming affects learning ability and survival in hatchling lizards"

In many animals, environmental stressors experienced in utero can produce substantial variation in the cognitive abilities of offspring. In reptiles, incubation temperatures experienced by embryos can influence hatchling brain function and learning ability.

Under climate warming, the eggs of some lizard species may experience higher temperatures, which could affect the cognitive abilities of hatchlings. Whether such changes in cognitive abilities influence the survival of hatchlings is unknown. To determine whether incubation-induced changes in spatial learning ability effect hatchling survival, we incubated velvet gecko, Amalosia lesueurii eggs at two fluctuating temperature regimes to mimic current (cold) versus future (hot) nest temperatures.