Data · dataset · 2022
Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>
Listed in SEDICI
The second messenger c-di-GMP is a ubiquitous molecule in bacteria that regulates diverse phenotypes.
Description
Among them, motility and biofilm formation are the most studied. Furthermore, c-di-GMP has been suggested to regulate virulence factors, therefore making it important for pathogenesis.
Previously we described that c-di-GMP regulates biofilm formation and swimming motility in Bordetella bronchiseptica. Here we present a multi-omic approach on B. bronchiseptica strains expressing different cytoplasmic c-di-GMP levels, including RNA-seq and shotgun proteomics with label-free quantification. We detected 64 proteins significantly up- or down-regulated in either low or high c-di-GMP levels and 358 genes differentially expressed between high c-di-GMP levels and the wild type strain.
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Among them we found stress-related proteins, nitrogen metabolism enzymes, phage-related genes and virulence factors. Interestingly, we observed that a virulence factor like the type three secretion system (TTSS) was regulated by c-di-GMP. B. bronchiseptica with high c-di-GMP levels showed significantly lower levels of TTSS components like Bsp22, BopN and Bcr4.
These findings were confirmed by independent methods as q-RT PCR and western blot. Higher intracellular levels of c-di-GMP correlated with impaired capacity to induce cytotoxicity in a eukaryotic cell in vitro and with attenuated virulence in a murine model. This work presents data that will support the role that the second messenger c-di-GMP plays in the pathogenesis of Bordetellae.
Links
Where it is published
- SEDICI record sedici.unlp.edu.ar/handle/10915/135429 ↗
landing page · from sedici unlp edu ar
- DOI doi.org/10.35537/10915/135429 ↗
DOI / persistent id · from sedici unlp edu ar
Catalogue records · 1
- OAI-PMH record sedici.unlp.edu.ar/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Asedi… ↗
metadata API · from sedici unlp edu ar
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Mass spectrometry 65% · RNA sequencing 65%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| SEDICI | oai:sedici.unlp.edu.ar:10915/135429 | 4 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | |
| concepts[field].local:field:engineering | mapping · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | |
| concepts[field].local:field:humanities | mapping · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | |
| concepts[field].local:field:social-science | mapping · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · sedici unlp edu ar | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:rna-seq | enrichment · sedici unlp edu ar | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | /metadata/dc/description |
| license | source · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | /metadata/dc/rights |
| publication_date | source · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | |
| title | source · sedici unlp edu ar | connector:sedici_unlp_edu_ar@1.0.0 | /metadata/dc/title |