OsterlundJBC_Figure 1

Pearson's correlation values were exported per cell in Cell Profiler, then minimum intensity thresholds were applied to ensure the cells were stained/expressing the protein(s) of interest. Here is the resulting summary where we exported the median and mode of each set of data. -Panel C, Images and histograms displayed of BMK-DKO cells expressing mCerulean3-BIK, where the folder name indicates the transfection ID (e.g. Venus-tagged BCL-XL = VBCLXL). -Panel D, FLIM-FRET data acquired for one biological replicate acquired 2017-01-11, in BMK-DKO cells expressing mCerulean3-tagged BIK (Donor).

Raw binding curve data was binned by Venus intensity. Here we provide the resulting binned data, and fitting parameters exported from GraphPad prism used to create the binding curves that were displayed in the paper. The remaining biological replicates are uploaded with "OsterlundJBC_SFigure 2".