<p>(A) Schematic representation of the DropMap workflow.</p>

After thawing, IgG-SC within each PBMC sample were analyzed by DropMap. Created in Biorender. <b>(B)</b> IgG secreting rate from (left) total IgG-SC or (right) IgG-SC binding at least one sE or sE-dimer of DENV1 or DENV2 from dengue infected cases (cumulative data of hospitalized and subclinical cases; n = 15) and healthy donors (HD; n = 3).

The number of DropMap experiments (assays) performed for each group is indicated. Horizontal bars represent median and interquartile range. Each dot represents one IgG-SC. <b>(C).</b> Gating strategy for the identification of CD19⁺ IgD^- IgM^- IgG⁺ cells and CD19⁺ IgD^- CD27⁺ CD38⁺⁺ ASC. <b>(D)</b> Percentages of CD19⁺ IgD^- IgM^- IgG⁺ cells and CD19⁺ IgD^- CD27⁺ CD38⁺⁺ plasmablasts in subclinical (blue) and hospitalized patients (red) at day 5 post RT-qPCR confirmed DENV infection, corresponding to day 7–8 after onset of symptoms.