Screening of cell cycle fusion proteins to identify kinase signaling networks

However, how kinases interact with the ubiquitin proteasome system (UPS) to elicit protein turnover is not fully understood. We sought a means of identifying kinase-substrate interactions to better understand signaling pathways controlling protein degradation.

Our prior studies used a luciferase fusion protein to uncover kinase networks controlling protein turnover. In this study, we utilized a similar approach to identify pathways controlling the cell cycle protein p27 Kip1 . We generated a p27 Kip1 -luciferase fusion and expressed it in cells incubated with compounds from a library of pharmacologically active compounds.