Omics · dataset · 2025
Immunohistochemistry, ploidy and next generation sequencing of ameloblastic carcinomas
Listed in UP Research Data Repository
<p dir="ltr">The histopathologic database of the Department of Oral and Maxillofacial Pathology at the University of Pretoria was searched for cases diagnosed as ameloblastic carcinoma (AC) over a 20-year period.
Description
Cases were subdivided into primary and secondary cases of AC. Ameloblastomas (ABs) that transformed into ACs and a control cohort of conventional ABs were also studied for comparative purposes.
Experienced Oral and Maxillofacial Pathologists reviewed all cases to confirm the diagnosis according to the latest World Health Organisation (WHO) diagnostic criteria. The original haematoxylin and eosin-stained slides, immunohistochemical (IHC) stains, and the best representative formalin-fixed paraffin-embedded (FFPE) tissue blocks were accessed. IHC analysis, including <i>Ki-67</i> proliferation index and surrogate stem cell markers <i>SOX2, OCT4</i>, and Glypican-3, was performed on the representative FFPE tissue block according to the manufacturer's instructions.
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High-resolution flow cytometry was used to analyse all cases to determine ploidy status. Molecular analysis utilising targeted next-generation sequencing (NGS) paired with an integrated sequencer was performed on all cases.</p>
Links
Where it is published
- DOI doi.org/10.25403/upresearchdata.29541785.v1 ↗
DOI / persistent id · from researchdata up ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from researchdata up ac za
Topics
- From keywords
- Earth & Environmental Science · Medicine & Health · Sequencing
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UP Research Data Repository | oai:figshare.com:article/29541785 | 20 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[modality].local:modality:sequencing | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Next generation sequencing'] |
| description | source · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | /metadata/dc/description |
| license | source · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| title | source · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | /metadata/dc/title |