Table 2_Viral metagenomic characterization of HAdV-B-related signals in bronchoalveolar lavage fluid from pediatric and adult clinical cohorts.xlsx

Adenoviridae-classified signals were more apparent in pediatric BALF libraries than in adult BALF libraries or environmental controls. Several pediatric BALF libraries showed broad read coverage across the HAdV-B-related representative contig, whereas adult libraries and environmental controls mainly showed low-coverage-breadth signals. Phylogenetic analysis placed BALF-derived penton base, hexon, and fiber sequences within HAdV-B-related lineages.

Twenty-seven capsid protein sequences from 12 pediatric samples had best BLASTp matches to references annotated as HAdV-B3 (n = 23) or HAdV-B7 (n = 4). These findings provide sequence evidence for HAdV-B-related sequence signals in clinically selected pediatric BALF samples and support further investigation using targeted assays and genome sequencing.</p>