← Search

Data · dataset · 2023

Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators

Listed in Teesside University Research Data Repository

Ferroptosis, an iron-dependent programmed cell death triggered by excessive lipid peroxidation, has shown promising therapeutic potentials in human diseases.

Description

Here, we describe a protocol of a CRISPR-Cas9 loss-of-function screen to identify regulators in response to different inducers of ferroptosis. We emphasize the steps of library amplification, drug treatment, high-throughput sequencing preparation, and bioinformatics analysis using Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK).

We also present a method to discover the regulators of ferroptosis and verify the potential targets efficiently.

Links

Where it is published

Catalogue records · 1

Topics

From keywords
Earth & Environmental Science
Inferred from text
Sequencing 75%
Provenance · 1 source records, 7 field assertions
SourceKeyLast seenRaw
Teesside University Research Data Repositoryoai:data.mendeley.com/65pfcj4j4m.119 h agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
concepts[field].local:field:earth-environmentalmapping · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
concepts[modality].local:modality:sequencingenrichment · researchdata tees ac ukkeyword-concept-rules@1.0.0title+description (75%)
descriptionsource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0/metadata/dc/description
licensesource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0/metadata/dc/rights
publication_datesource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
titlesource · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0/metadata/dc/title