[CLA Phosphate Threshold Data] - Measurements of C–P lyase phosphate thresholds in Stutzerimonas frequens cultures (North Pacific Methane Project) (Collaborative Research: NSF OCE-BSF: Coupling organic nutrient cycling to methane production in the oligotrophic North Pacific Ocean)

The dataset includes measurements of total dissolved phosphorus (TDP), soluble reactive phosphorus (SRP), and dissolved organic phosphorus (DOP) in the culture medium at the end of incubation. Additional parameters include cell concentrations from cultures, methane produced by the cultures during the experiment, and rates of C-P lyase activity (CLA).

TDP, SRP, and DOP were measured using standard colorimetric methods. Cell concentrations were determined by flow cytometry. Methane concentrations and δ¹³C values were measured with a Picarro gas analyzer, and CLA was quantified using a fluorescently labeled phosphonate probe.