Venom on ice: Novel venoms from Antarctic marine animals.

This integrative project aims to investigate the origin and evolution of secreted proteins in the venom glands of toxic polar animals by means of: - Analysis of mechanisms of evolution in multigene families. - Phylogenetic analysis of evolutionary relationships among secreted proteins in the venom glands of major lineages; - Search for correlations between: (i) evolution of venom gland structure (ii) molecular evolution of venom components, and (iii) ecological specialisation of the animal - Bioactivity studies will be conducted upon representative purified or synthesised proteins. - A first ever comparison of the convergent strategies between Arctic and Antarctic endemic fauna.

The results will help us to understand protein evolution, will cast light on the classic problem of how venom systems evolve, and may provide leads in the search for commercially-exploitable venom proteins. Taken from the 2008-2009 Progress Report: Progress against objectives: We have completed the genetic analyses of the specimens and sequence analyses. Phylogenetic positioning is robust other than a few deep level nodes.

We are undertaking a second round of genetic analyses using different primers in order to resolve these nodes. Biochemical analyses of crude protein secretions from the posterior salivary (venom) glands has revealed temperature specific modifications of some of the venom components to adapt them to the polar conditions. We have tested the secretions in a battery of assays.

We are now repeating those assays using purified proteins in order to determine which types are responsible for particular effects and also investigate synergistic interactions. Taken from the 2009-2010 Progress Report: Progress against objectives: We have undertaken genetic analyses of the specimens collected, and investigated specific adaptations of their venom systems. Results to-date include: - Antarctic octopuses are more genetically diverse than previously appreciated, including at least one new genus - an inverse relationship exists between the size of the venom gland and the size of the beak - their venoms have undergone temperature-specific adaptations