Table 1_Whole-genome sequencing and biocontrol functional analysis of Pseudomonas chlororaphis XZL-8 for controlling poplar canker pathogen Cytospora chrysosperma.docx

This study aimed to characterize the taxonomic status, genomic features and biocontrol potential of strain XZL-8.</p>Methods<p>Polyphasic identification and whole-genome sequencing were conducted to confirm the species of strain XZL-8.

Functional annotation against NR, COG, GO and KEGG databases and antiSMASH secondary metabolite prediction were carried out to decode its functional genes. A series of in vitro antagonistic experiments, as well as detached branch inoculation assays, were implemented to verify its phenotypic traits.</p>Results<p>Strain XZL-8 was classified as Pseudomonas chlororaphis subsp. aureofaciens. A total of 22 secondary metabolite biosynthetic gene clusters were predicted in its genome; the gene clusters encoding hydrogen cyanide and pyrrolnitrin synthesis shared high homology with known reference clusters, with similarity coefficients reaching 0.85 and 0.99, respectively.