Differential effects of chronic Limb ischemia on gene profile changes between rat gastro and soleus muscles

Gene set enrichment analysis further highlighted fiber composition differences by shBulk RNAseq discovered that over 1800 DEGs were revealed when comparing IFCO soleus and gastroc with the most downregulated DEGs in the gastroc. A total of 816 DEGs were identified between IFCO and sham solei, with 730 upregulated and 86 downregulated genes. Conversely, only 89 DEGs were identified between the IFCO and sham gastrocs, with all but 3 being upregulated.owing upregulation of glycolytic genes, such as glucose phosphate isomerase (GPI), Lactate dehydrogenase A (LDHA), and 6-phosphofructokinase, muscle type (PFKM), within the gastroc, while the soleus upregulated Lactate dehydrogenase B (LDHB) and NADPH oxidase 1 (NOX1).

The soleus also has unregulated NADPH dehydrogenase quinone 1 (NQO1), Glutathione peroxidase 1 (GPX1), and RNA Binding Motif Protein 11 (RBM11), consistent with other reports stating that type I fibers possess higher anti-oxidative capacity. In total, almost 1700 DEGs were identified between the sham muscles, with most DEGs being downregulated in the gastroc compared to the soleus.