Single-nucleus transcriptome datasets of the ligular region at preligule band and ligule outgrowth stages in 12-day-old maize B73 seedlings

For each stage, tissues were dissected from 30 seedlings, and nuclei were isolated using a modified Galbraith buffer‑based protocol. The crude nuclear suspension was purified by fluorescence‑activated cell sorting (FACS) to enrich intact nuclei.

Approximately 20,000 purified nuclei from each sample were loaded onto the 10x Genomics Chromium Controller, and libraries were constructed using the Chromium Next GEM Single Cell 3' Kit v3.1. Sequencing was performed on an Illumina NovaSeq 6000 platform with 150‑bp paired‑end reads. This dataset enables high‑resolution transcriptomic analysis of ligule development, providing a valuable resource for studying cell‑type‑specific gene expression dynamics in maize.