BXSB_mice_CD20_BZR_repertoire, Werner et al

We could show that male BXSB mice treated with an anti-CD20 monclonal antibody show reduced diversity for IgG VDJ recombinations. To perform this analysis Next Generation Sequencing was performed by on the Illumina MiSeq platform using a pair of overlapping paired-end reads (2x300 bp). 20 % PhiX was added for improved performance.

Paired-end reads were merged with the IgRepertoireConstructor (Safonova et al., 2015). Read Quality was ensured by FASTQC quality analysis by MultiQC (Ewels et al., 2016). Molecular amplification fingerprinting (MAF) error correction was achieved with unique molecular identifiers (UMI) as part of the cDNA synthesis and Multiplex Primers.