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Structure · dataset · 2019

MC3T3-E1 single cell qPCR results (Biomark, Fluidigm)

Listed in Teesside University Research Data Repository

Fluidigm Biomark qPCR raw data was pre-processed using Fluidigm Real-Time PCR Analysis software.

Description

Failed assays were removed. The default quality threshold cutoff (0.65) was used to identify potential artifacts.

The baseline correction was to linear, and the Ct threshold detection method was set to Auto (Detectors). The Fluidigm Singular Analysis Toolset v3.6.2 was used to remove outliers using the IdentifyOutliers() command. Cell types used for this study: Wildtype MC3T3-E1 at differentiation stages T0, T3, T10, T14 and T21, Satb2-mutant cell lines C8 at T0 and C9 and T0 and T14.

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Where it is published

Catalogue records · 1

Topics

From keywords
Earth & Environmental Science · Materials Science
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concepts[field].local:field:earth-environmentalmapping · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
concepts[field].local:field:materials-sciencemapping · researchdata tees ac ukconnector:researchdata_tees_ac_uk@1.0.0
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