Data · dataset · 2026
Chemical and Proteomic Characterization of Bioactive Protein-Enriched Fraction from Schizophyllum commune with Anticancer Activity in HeLa Cells
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.1021/acsomega.6c03766.s002
Schizophyllum commune, commonly known as the split gill mushroom, is an edible fungus recognized as a source of bioactive compounds with potential applications in the food and biotechnology industries.
Description
In this study, protein-enriched fractions were isolated from the fruiting bodies of <i>S. commune</i> using ion-exchange chromatography coupled with a bioassay-guided approach. Cytotoxic activity was evaluated in HeLa cells using MTT and colony formation assays.
Among the fractions, the protein-enriched fraction F2 exhibited antiproliferative activity with an IC<sub>50</sub> value of 0.37 μg/mL and significantly inhibited colony formation, while showing minimal effects on normal HaCaT cells. Flow cytometry and fluorescence staining further supported the involvement of apoptosis in F2-treated HeLa cells. Chemical and proteomic characterization of the protein-enriched fraction F2 by SDS-PAGE and LC–MS/MS identified 64 protein groups.
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Gene ontology analysis revealed proteins involved in diverse biological processes, while physicochemical profiling demonstrated a heterogeneous protein composition with isoelectric points ranging from 4 to 7. These findings provide insight into the chemical and proteomic composition of protein-enriched fraction F2. The results support further investigation of <i>S. commune</i>-derived protein-enriched fractions as a source of bioactive proteins, particularly in bioactivity-guided fractionation studies.
Links
Where it is published
- DOI doi.org/10.1021/acsomega.6c03766.s002 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Cancer · Cancer · Cancer · Chemistry · Chemistry · Chemistry · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences · Mass spectrometry · Mass spectrometry · Mass spectrometry · Medicine & Health · Medicine & Health · Medicine & Health
- Inferred from text
- Medicinal and biomolecular chemistry 71%
Related
Provenance · 3 source records, 24 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34041474 | 10 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34041474 | 10 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34041474 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[field].anzsrc:group:3404 | enrichment · zivahub uct ac za | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].local:field:chemistry | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['lc – ms'] |
| concepts[modality].local:modality:mass-spectrometry | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['lc – ms'] |
| concepts[modality].local:modality:mass-spectrometry | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['lc – ms'] |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |