Sequencing data — [Metagenomic Detection and Customized Bioinformatic Analysis]

Upon completion of sequencing, the raw data were first processed through procedures such as splitting and quality trimming to obtain high-quality sequences termed valid data. Assembling software was used for de‑novo assembly of these valid sequences, followed by gene prediction.

The predicted genes were annotated and classified at both taxonomic and functional levels against databases including NR, KEGG and eggNOG. Meanwhile, the relative abundance of species in each sample was calculated. Based on these results, further analyses including sample similarity clustering, ordination test and differential statistical comparison were carried out.