<p>Lack of glycation in the LD spirochete <i>B. mayonii</i> and RF <i>Borrelia</i> spp.</p>

<p>(A) <i>B. mayonii</i> was grown to ~7 × 10⁷ cell ml^-1 before collection by centrifugation and resuspension in RPMI (0), RPMI with 100, 500 or 2000 µM glycerol or 500 µM mannose (man), maltose (malt), trehalose (tre) or <i>N</i>-acetyl glucosamine (GlcNAc) or 100 µM methylglyoxal (MG) and incubation for 24 h at 35°C. Equal amounts of whole cell lysates were separated by SDS-PAGE and glycation was visualized by immunoblot analyses with anti-MG antibodies; anti-FlaB antibodies were used as a loading control.

(B) Whole cell lysates of <i>B. burgdorferi</i> and <i>B. mayonii</i> grown in BSK + RS were separated by SDS-PAGE and MgsA levels were visualized by immunoblot analyses with anti-MgsA antibodies; anti-FlaB antibodies were used as a loading control. (C) <i>B. burgdorferi</i>, <i>B. hermsii</i> and <i>B. parkeri</i> were grown to ~7 × 10⁷ cell ml^-1 before collection by centrifugation and resuspension in RPMI (-), RPMI with 100 µM glycerol (gly) or 100 µM methylglyoxal (MG) and incubation for 24 h at 35°C. Equal amounts of whole cell lysates were separated by SDS-PAGE and protein glycation was measured by immunoblot analyses with anti-MG antibodies.