Supplementary Annexes to the Doctoral Thesis: Contribution to the Breeding of <i>Stevia rebaudiana</i> (Bert.) through the Integration of Conventional and Biotechnological Techniques in Colombia

<p dir="ltr"><b>Supplementary annexes to the doctoral thesis “Contribution to the breeding of </b><b><i>Stevia rebaudiana</i></b><b> (Bert.) through the integration of conventional and biotechnological techniques in Colombia”, submitted in fulfilment of the requirements for the degree of Doctor of Agricultural Sciences at the Universidad Central “Marta Abreu” de Las Villas, Cuba.</b></p><p dir="ltr">This document compiles the experimental, analytical and documentary evidence supporting the main components of the doctoral research.

It includes: agromorphological characterisation of segregating lines derived from <i>Stevia rebaudiana</i> cv. ‘Morita II’; field experimental design and UPOV-based descriptors; molecular characterisation using SSR markers, including DNA extraction, PCR optimisation, electrophoretic profiles and curation of the final multilocus matrix; physiological and biochemical data, including steviol glycoside quantification and chromatographic records; and the experimental and statistical support for the <i>in vitro</i> propagation protocol, covering establishment, shoot multiplication and rooting.