Posterior explants of Stage 26 embryos treated with picrotoxins

Explants were sectioned posterior to the otic vesicle and treated with 0.5 mM picrotoxin for 3 h to inhibit Gamma-Aminobutyric Acid (GABA) signaling, followed by removal of the ventral yolk. Two biological replicates were prepared for each condition, with each replicate pooling explants from three embryos.

RNA-seq was performed to identify transcriptional changes associated with GABA signaling during tailbud-stage body axial elongation. The dataset includes raw FASTQ files and metadata, enabling the analysis of differential gene expression patterns under GABA inhibition.