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Data · dataset · 2026

<p>Representative deconvolved Z-stack optical sections of <i>T. cruzi</i> epimastigotes stained for (A) hexokinase (HK), (B) citrate synthase (CS), and (C) glycerol kinase (GK), together with H2B.</p>

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<p>V-Myc and DAPI.

Description

HK, CS, and GK were detected with Alexa Fluor 488-conjugated secondary antibody, H2B.V-Myc was detected with Alexa Fluor 555-conjugated secondary antibody, and DNA was stained with DAPI. Images were acquired using an Olympus BX51 fluorescence microscope equipped with a 100 × oil-immersion objective and controlled by Olympus cell^R software.

Z-stacks were acquired as sequential optical sections through the parasite body and deconvolved before visualization. Bright-field, Alexa Fluor 488, Alexa Fluor 555, DAPI, and merged channels are shown. Scale bars, 100 µm.</p> <p>(TIF)</p>

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