<p>Primary keratinocytes demonstrate dose-dependent gene induction via IFNγ-treatment.</p>

Data is represented as ΔCt, which is calculated by subtracting the Ct value of β-actin from the Ct of induced genes. All Ct values were acquired directly from qPCR with a maximum Ct threshold of 35. <b>(B)</b> Fold change in <i>CXCL10, CCL5, IL15, IL12A</i>, and <i>IFI16</i> expression in primary keratinocytes (n = 10) 4 hours following mock-treatment or treatment with 0.1, 1.0, 10, or 100 U/mL IFNγ.

Fold change is calculated as relative to mock expression, normalized to β-actin, using the ΔΔCt method. The bold line represents the average fold change of gene expression across all donors. Dashed lines represent fold change expression in individual donors. <b>(C)</b> Fold change in <i>CXCL10, CCL5, IL15, IL12A</i>, and <i>IFI16</i> expression in primary keratinocytes mock-treated or treated with 100 U/mL IFNγ at 4 hours and 24 hours post-treatment.