Direct Molding of Luer Connections in PDMS MicrofluidicDevices: Comparison with Conventional Interface Methods The document contains pressure test data, test results using thicker chips, which were added in the “Supplementary” section, and tests conducted using biofilm as a biological model.

In this work, we present a simple, low-cost, and reproducible method to directly mold male Luer cone fittings into PDMS devices during fabrication, without compromising the geometry of microchannels. To evaluate the effectiveness of this approach, we compared the performance of three connection types—PTFE tubing, metal pipes, and molded Luer adaptors—by measuring the infusion pressure at which fluid leakage occurred.

Devices with integrated Luer adaptors consistently withstood higher pressures (up to 1555 mbar) with reduced variability across repeated connection cycles, while PTFE and metal connections failed at lower pressures and showed inconsistent leakage behavior. As a biological proof of concept, Staphylococcus aureus biofilms were grown in the microfluidic channels under continuous perfusion and subsequently exposed to flow rates of 50, 83.3, and 116.7 µL/min.