Data · dataset · 2026
Analysis of Transcriptome Data from LSDV-Infected MAC-T Cells
Listed in ScienceDB
Transcriptome sequencing was done by Megi Bio (Shanghai).
Description
Total RNA was extracted from cells using TRIzol reagent (Invitrogen) according to the manufacturer 's instructions. RNA was treated with DNase I for 30 min at 37 ° C to remove residual DNA.
Qualified samples were used for library construction. Double-end sequencing libraries were prepared using the ABclonal mRNA-seq Lib Prep Kit (ABclonal, China) according to the manufacturer 's instructions. Library quality was assessed using the Agilent Bioanalyzer 4150 system.
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Finally, libraries were sequenced on an Illumina NovaSeq 6000 (or MGISEQ-T7) to generate 150 bp double-ended sequencing data. Bioinformatic analysis was performed using data generated from the Illumina (or BGI) platform.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.37308 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Medicine & Health · Social Science
- Inferred from text
- Bioinformatics and computational biology 70% · RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.37308 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3102 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (70%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |