Nuclear IL-33 plays an important role in the suppression of filaggrin, loricrin, keratin 1, and keratin 10 by IL-4 and IL-13 in human keratinocytes

The data in Fig. 2 showed that nuclear IL-33 is involved in the downregulation of differentiation markers. The data in Fig. 3 showed that Nuclear IL-33 contributes to the downregulation of FLG, LOR, KRT1, and KRT10 by regulating the nuclear translocation of STAT3 and STAT6 The data in Fig. 4 showed that Nuclear IL-33 functions as a transcription cofactor of STAT3, facilitating p-STAT3 to the FLG promoter thereby inhibiting FLG transcription.

The data in Fig. 5 showed that IL-4/IL-13 decreases the expression of RunX1 through activating STAT3 and STAT6. The data in Fig. 6 showed that Nuclear IL-33 participates in the downregulation of LOR, KRT1, and KRT10 by reducing the RunX1 level. The data in Fig. S1 showed that Both IL-4 and IL-13 increase IL-33 expression, which is required for the downregulation of FLG, LOR, KRT1, and KRT10.