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Data · dataset · 2026

<p>Validation of heterologous antibodies used for immunofluorescence detection of metabolic enzymes in <i>T. cruzi.</i></p>

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<p>(A) BLASTP analysis showing the closest <i>T. cruzi</i> orthologs of the <i>T. brucei</i> proteins used as antigens for antibody production.

Description

Protein length, query coverage, sequence identity, and E-value are shown for citrate synthase, glycerol kinase, and hexokinase. (B) Western blot analysis of <i>T. cruzi</i> protein extracts using antibodies raised against <i>T. brucei</i> citrate synthase (CS), hexokinase (HK), and glycerol kinase (GK).

Arrows indicate bands compatible with the expected molecular masses of the corresponding <i>T. cruzi</i> proteins.</p> <p>(TIF)</p>

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Provenance · 1 source records, 14 field assertions
SourceKeyLast seenRaw
ZivaHuboai:figshare.com:article/3401770712 d agoJSON v1
FieldAssertionExtractorEvidence
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