TMT proteomics of erythroblasts from healthy donors and erythroblasts/ring sideroblasts from MDS-RS patients

The objective of this data collection was to assess several parameters on how the bone marrow of MDS-RS patients differs from that of healthy donors. The samples included in this analyses were 3 samples of sorted GPA+ erythroblasts pooled from 5 healthy donors (2+2+1) and 3 samples of sorted GPA+ erythroblasts plus 3 samples of purified ring sideroblats from 3 MDS-RS patients.

This dataset includes the raw mass spectra, QC and processed results. Processing: In short, FACS-separated samples were snap frozen in liquid nitrogen. Cell pellets were lysed with 4 % SDS lysis buffer and prepared for mass spectrometry using a modified version of the SP3 protein clean up and digestion protocol published by Moggridge S. et al 2018.