Table · dataset · 2026
Supplemental Material For: Mesenchymal stromal cell derived membrane particles improve metabolic activity and functional performance during porcine kidney normothermic machine perfusion
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<p dir="ltr"><b>Introduction</b></p><p dir="ltr">Kidneys from donation after circulatory death (DCD) donors are increasingly used to alleviate organ shortage but highly vulnerable to ischemia-reperfusion injury, leading to poor graft outcomes.
Description
Normothermic machine perfusion (NMP) enables targeted intervention during donor kidney preservation. Membrane particles (MPs) derived from mesenchymal stromal cells (MSCs) have shown regenerative potential.
We aimed to investigate MPs as a potential therapeutic strategy to improve metabolism and function in porcine DCD kidneys during NMP.</p><p dir="ltr"><b>Methods</b></p><p dir="ltr">Six pairs of porcine kidneys underwent 4-hour NMP. Within each pair, one kidney received MPs derived from 1.0×10<sup>7</sup>MSCs isolated from the abdominal subcutaneous adipose tissue of healthy donors, while the contralateral kidney served as a control.
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Hemodynamics, sodium reabsorption, oxygen consumption, and injury markers were monitored. Gene expression related to inflammation and metabolism were analyzed.</p><p dir="ltr"><b>Results</b></p><p dir="ltr">MP administration did not affect renal blood flow or intrarenal resistance. Injury markers (AST, LDH,<i>IL-6</i>) were increased over time in both groups, without significant difference between MP-treated and control kidneys.
In contrast, MP-treated kidneys showed significantly higher expression of metabolic genes (<i>PDHB</i>,<i>ATP6V0D2</i>,<i>NDUFS8</i>). Functionally, MP administration enhanced sodium reabsorption, urinary creatinine excretion, and oxygen utilization efficiency compared with controls.</p><p dir="ltr"><b>Conclusion</b></p><p dir="ltr">MSC-derived MPs can be safely delivered to porcine kidneys and promote kidney functional performance and metabolic activity.
These findings support MPs as a promising cell-free therapeutic strategy to improve the quality of kidneys during NMP.</p><p dir="ltr"><br><br></p>
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Where it is published
- DOI doi.org/10.6084/m9.figshare.34002339.v1 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
Provenance · 3 source records, 16 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| HKU DataHub · removed upstream | oai:figshare.com:article/34002339 | 13 d ago | JSON v1 |
| figshare | oai:figshare.com:article/34002339 | 13 d ago | JSON v1 |
| Loughborough Research Repository · removed upstream | oai:figshare.com:article/34002339 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
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| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
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| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
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| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
| license | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare com | connector:figshare_com@1.0.0 | |
| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |