Data · dataset · 2021
IP STING - Identification of new partners
Listed in Teesside University Research Data Repository
To access STING function in absence of inflammation, given the tight interconnexion between metabolic and immune pathways, we questioned the impact of Sting ablation on metabolic parameters in vivo.
Description
Mice deficient for STING presented profound modifications of glucose management and body temperature. To identify the molecular mechanism through which Sting regulates metabolic homeostasis, we performed tandem-affinity purification of Flag- and HA-tagged Sting (F/HA-Sting) stably expressed in mouse embryonic fibroblasts (MEF) knockout for Sting (MEF Sting-/- ).
Immunopurified material was analyzed by Mass Spectrometry to identify Sting protein partners.
Links
Where it is published
- DOI doi.org/10.17632/23drspzh3s.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science
- Inferred from text
- Mass spectrometry 75%
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/23drspzh3s.1 | 14 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |